Artikel
Profiling PUFAs and PUFA-derived metabolites in polytraumatic patients
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Autoren
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Moujie Rang
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Madlen Reinicke
- University of Leipzig, Institute for Laboratory Medicine, Leipzig, Germany
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Uta Ceglarek
- University of Leipzig, Institute for Laboratory Medicine, Leipzig, Germany
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Leyu Zheng
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Carolin Fuchs
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Annette Keß
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Christian Kleber
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Georg Osterhoff
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
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Gabriela Aust
- University of Leipzig, Research Laboratory of Trauma, Leipzig, Germany
| Veröffentlicht: | 23. Oktober 2023 |
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Gliederung
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Objectives: Oxylipids, potent mediators having pro-inflammatory or resolving properties, result from the degradation of arachidonic acid (AA) and related polyunsaturated fatty acids (PUFA). Here, we examined whether circulating PUFA-derived metabolites are potential biomarkers for the post-traumatic time course and outcome of traumatized patients.
Methods: We profiled PUFAs and their metabolites in 36 patients, showing a wide range of injury severity scores (ISS, 5–75), up to 240 h after injury by targeted liquid chromatography-tandem mass spectrometry and related them to patient’s clinical data.
Results and conclusion: The PUFA levels varied obviously immediately after trauma, independent of the ISS, likely reflecting dietary intake and synthesis, and declined in almost all patients during hospitalization. Consistently, especially in the early post-traumatic phase, the metabolite profiles are also heterogeneous in most patients. Several metabolites distinguish severe-injured patients (ISS>=25, n=14) from the other patient groups (ISS<16, n=11; ISS 16-24, n=11). Among the AA-derived metabolites 6-keto-PGF1a and tetranor-12(S)-HETE were higher 1–8 h after trauma in severe-injured patients. In this group the levels of various dihydroxyeicosatrienoic acids (DHETs), the stable derivatives of bioactive epoxyeicosatrienoic acids (EETs), suggested to be protective, are lower 48–240 h after injury compared to the other patients. Lowest levels or the most distinct decrease of DHETs are seen in patients with long intensive care unit stay or fatal outcome in the late post-operative phase.
In conclusion, some PUFA-derived metabolites are potential biomarkers in multi-injured patients.
